Methylation of F2RL3, CDKN2A genes and sudden cardiac death
https://doi.org/10.52727/2078-256X-2021-17-4-6-10
Abstract
The aim of the study was to evaluate the association of methylation of the F2RL3, CDKN2A gene with sudden cardiac death (SCD). Material and methods. Case-control study design. The SCD group included 150 deceased men (mean age 46.7 ± 9.2 years) with the main pathological diagnoses of acute circulatory failure, acute coronary insufficiency, which meets the SCD criteria of the European Society of Cardiology. The control group included 150 men who died suddenly, but not due to cardiovascular pathology (mean age 42.6 ± 1.2 years). DNA was isolated by phenol-chloroform extraction from myocardial tissue in both groups. The methylation status of the F2RL3 gene (19: 16890405-16890606, GRCh38.p13) and the CDKN2A gene (9: 21974726-21974877, GRCh38.p13) was assessed by methyl-specific polymerase chain reaction. Results. In the SCD group, 17.3 % (26/150) had the F2RL3 gene completely methylated (MM); in 6.0 % (9/150) it is completely unmethylated (UU); 76.7 % (115/150) had both methylated and unmethylated F2RL3 (MU) gene. In the control group, 16 % (24/150) had the F2RL3 gene completely methylated (MM); in 5.3 % (8/150), it is completely unmethylated (UU); 78.7 % (118/150) had both methylated and unmethylated F2RL3 (MU) gene. When comparing the groups, there were no statistically significant differences in the methylation status of the F2RL3 gene between the groups (p > 0.05). In all subjects in the SCD group and the control group, the CDKN2A gene is completely unmethylated. Conclusions. Methylation of genes F2RL3, CDKN2A is not associated with sudden cardiac death.
Supporting agencies: The reported study was funded by RFBR and Novosibirsk region according to the research project N 19-415- 543001.
About the Authors
A. A. Ivanova
Research Institutе of Internal and Preventive Medicine, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Russian Federation
Russian Federation
Anastasiya A. Ivanova, candidate of medical sciences, senior researcher at the laboratory of molecular genetic studies of therapeutic diseases
630089, Novosibirsk, Boris Bogatkov str., 175/1
A. A. Gurazheva
Research Institutе of Internal and Preventive Medicine, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Russian Federation
Russian Federation
Anna A. Gurazheva, junior researcher, laboratory of molecular genetic research of therapeutic diseases
630089, Novosibirsk, Boris Bogatkov str., 175/1
S. V. Maksimova
Novosibirsk State Medical University
Russian Federation
Russian Federation
Sofya V. Maksimova, 5th year student of the Faculty of Pediatrics
630091, Novosibirsk, Krasnyj av., 52
S. K. Malyutina
Research Institutе of Internal and Preventive Medicine, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Russian Federation
Russian Federation
Sofya K. Malyutina, doctor of medical sciences, professor, head of the laboratory of etiopathogenesis and clinic of internal medicine
630089, Novosibirsk, Boris Bogatkov str., 175/1
V. P. Novoselov
Novosibirsk Regional Office of Forensic Medical Examination
Russian Federation
Russian Federation
Vladimir P. Novoselov, doctor of medical sciences, professor
630087, Novosibirsk, Nemirovich-Danchenko str., 134
V. N. Maksimov
Research Institutе of Internal and Preventive Medicine, Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences
Russian Federation
Russian Federation
Vladimir N. Maksimov, doctor of medical sciences, professor, head of the laboratory of molecular genetic studies of therapeutic diseases
630089, Novosibirsk, Boris Bogatkov str., 175/1
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Review
For citations:
Ivanova A.A., Gurazheva A.A., Maksimova S.V., Malyutina S.K., Novoselov V.P., Maksimov V.N. Methylation of F2RL3, CDKN2A genes and sudden cardiac death. Ateroscleroz. 2021;17(4):6-10. (In Russ.) https://doi.org/10.52727/2078-256X-2021-17-4-6-10
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