Analysis of the association of the common variant rs13107325 of the divalent cation transporter gene SLC39A8 with the lipid profile among Novosibirsk adolescents

Violation of lipid metabolism is one of the main risk factors for the atherosclerosis in humans. In the course of genome-wide association studies, dozens of gene variants have been identified, to be responsible for predisposition to dyslipidemias. However, many of the associations are either not confirmed by replication or turn out to be specific for certain populations. The aim of the study was to assess the prevalence of one of the most pleiotropic polymorphisms of the human genome – rs13107325 – in a population sample of adolescents in Novosibirsk and to analyze its association with lipid metabolism. The study used blood samples and data from examinations of 1582 adolescents collected during a standardized medical examination at the Institute of Internal and Preventive Medicine – branch of ICG SB RAS. Genotyping for rs13107325 of the SLC39A8 gene was carried out using real-time PCR. A one-way ANOVA was used to assess the correlation of genotypes with lipid levels and body mass index. It was shown that the frequencies of the rs13107325 variant among whites of Western Siberia are lower than the European ones (p = 0.05 ± 0.004). An association with lipid metabolism (total cholesterol, triglyceride and high-density lipoprotein cholesterol level) as well as with body mass index was not confirmed either overall or in any of the groups differing in sampling periods (contrasting in the average food intake). This may indicate that the contribution of the rs13107325 variant to dyslipidemia in adolescents in Western Siberia is insignificant, and the average food intake does not affect the penetrance of rs13107325 in relation to lipid metabolism disorders and body mass index.

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