Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats
https://doi.org/10.15372/ATER20200402
Abstract
This work presents the characteristics of the catalytic activity of hydroxylation of benzo[a] pyrene (B[a]P) in rat liver microsomes using plasma lipoproteins (LP) (VLDL, LDL, HDL) as transport forms of B[a]P. The aim of the study was: to study the effect of the LP-component of the LP-B[a]P complexes on the rate of B[a]P hydroxylation in rat liver microsomes. The studies were carried out on the microsomal fraction of rat liver using B[a]P as a substrate, ultracentrifugation of individual fractions of VLDL, LDL, HDL plasma, and spectrofluorimetric determination of the activity of arylhydrocarbonhydroxylase. In work on microsomes of rat liver the rate of hydroxylation of B[a]P in free form and in the form of B[a]P complexes with various fractions of LP was analyzed. The analysis showed that the arylhydrocarbonhydroxylase activity (pmol 3-OH-B[a]P in 1 min per 1 mg of protein) decreases in the complex form B[a]P with LP. So for transport form B[a]P with HDL the decrease was by 22%, for transport form B[a]P with VLDL the decrease was 30%, and for transport form B[a]P with LDL it was 52%. It should be noted that Km for the substrate remained practically unchanged in all forms. The Vmax and Km values indicate that the LP component in complexes with B[a]P can be a noncompetitive inhibitor of B[a]P hydroxylation in rat liver microsomes.
About the Authors
L. M. Polyakov
Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine
Russian Federation
630117, Novosibirsk, Timakov str., 2
Russian Federation
630117, Novosibirsk, Timakov str., 2
R. A. Knyazev
Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine
Russian Federation
630117, Novosibirsk, Timakov str., 2
Russian Federation
630117, Novosibirsk, Timakov str., 2
N. V. Trifonova
Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine
Russian Federation
630117, Novosibirsk, Timakov str., 2
Russian Federation
630117, Novosibirsk, Timakov str., 2
M. V. Kotova
Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine
Russian Federation
630117, Novosibirsk, Timakov str., 2
Russian Federation
630117, Novosibirsk, Timakov str., 2
E. I. Solovyova
Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine
Russian Federation
630117, Novosibirsk, Timakov str., 2
Russian Federation
630117, Novosibirsk, Timakov str., 2
A. V. Ryabchenko
Research Institute of Biochemistry of Federal Research Center of Fundamental and Translational Medicine
Russian Federation
630117, Novosibirsk, Timakov str., 2
Russian Federation
630117, Novosibirsk, Timakov str., 2
References
1. Saikia J., Khare P., Saikia P., Saikia B.K. Polycyclic aromatic hydrocarbons (PAHs) around tea processing industries using high-sulfur coals. Environ. Geochem. Health, 2017; 39 (5): 1101–1116. doi: 10.1007/s10653-016-9879-0
2. Li Y.C., Qiu J.Q., Shu M., Ho S.S.H., Cao J.J., Wang G.H., Wang X.X., Zhao X.Q. Environ. Sci. Pollut. Res. Int., 2018; 25 (5): 4750–4760. doi: 10.1007/s11356-
3. -0603-0
4. Moffat I., Chepelev N., Labib S., Bourdon-Lacombe J., Kuo B., Buick J.K., Lemieux F., Williams A., Halappanavar S., Malik A., Luijten M., Aubrecht J., Hyduke D.R., Fornace A.J. Jr, Swartz C.D., Recio L., Yauk C.L. Comparison of toxicogenomics and traditional approaches to inform mode of action and points of departure in human health risk assessment of benzo[a] pyrene in drinking water. Crit. Rev. Toxicol., 2015; 45 (1): 1–43. doi: 10.3109/10408444.2014.973934
5. Castelli F., Librando V., Sarpietro M.G. Calorimetric approach of the interaction and absorption of polycyclic aromatic hydrocarbons with model membranes. Environ. Sci. Technol., 2002; 36: 2717–2723. doi: 10.1021/es010260w
6. Verma N., Pink M., Boland S., Rettenmeier A.W., Schmitz-Spanke S. Benzo[a]pyrene-induced metabolic shift from glycolysis to pentose phosphate pathway in the human bladder cancer cell line RT4. Sci. Rep., 2017; 7 (1): 9773. doi: 10.1038/s41598-017-09936-1
7. Shu H.P., Bymun E.N. Systemic excretion of benzo(a) pyrene in the control and microsomally induced rat: the influence of plasma lipoproteins and albumin as carrier molecules. Cancer Res. 1983; 43 (2): 485–490.
8. Поляков Л.М., Князев Р.А., Рябченко А.В., Котова М.В., Трифонова Н.В. Липопротеины крови как платформа для транспорта гидрофильных и гидрофобных соединений. Сиб. науч. мед. журн., 2019; 39 (4): 30–36. doi: 10.15372/SSMJ20190404
9. Busbee D.L., Norman J.O., Ziprin R.L. Comparative uptake, vascular transport, and cellular internalization of aflatoxin-B1 and benzo(a)pyrene. Arch. Toxicol., 1990; 64: 285–290. doi: 10.1007/bf01972988
10. Brown M.S., Goldstein J.L. The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor. Cell, 1997; 89: 331–340. doi: 10.1038/41672
11. Kazuhiro S., Kawanishi M., Yagi T. Modulation of benzo[a]pyrene-DNA adduct formation by CYP1 inducer and inhibitor. Genes Environ., 2017; 39: 14. doi: 10.1186/s41021-017-0076-x
12. Uppstad H., Оvreba S., Haugen A., Mollerup S. Importance of CYP1A1 and CYP1B1 in bioactivation of benzo[a]pyrene in human lung cell lines. Toxicol. Lett., 2010; 192 (2): 221–228. doi: 10.1016/j.toxlet.2009.10.025
13. Reed L., Jarvis I.W., PhillipsD.H., Arlt V.M. Enhanced DNA adduct formation by benzo[a]pyrene in human liver cells lacking cytochrome P450 oxidoreductase. Mutat. Res., 2020; 852: 503162. doi: 10.1016/j.mrgentox
14. Hatch F.T., Lees R.S. Practical method for plasma lipoprotein analysis. Adv. Lipid Res., 1968; 6: 2–68.
15. Schenkman J.B., Jansson I. Measurement of cytochrome P-450. Curr. Protoc. Toxicol., 2002; Chapter 4: Unit4.1. doi: 10.1002/0471140856.tx0401s13
16. Polyakov L.M., Chasovskikh M.I., Panin L.E. Binding and treatment of benzo(a)pyrene by blood plasma lipoproteins: The possible role of apolipoprotein B in this process. Bioconjug. Chem., 1996; 7 (4): 396–400. doi: 10.1021/bc960005e
17. Souès S., Fernandez N., Souverain P., Lesca P. Intracellular lipoproteins as carriers for 2,3,7,8- tetrachlorodibenzo-p-dioxin and benzo(a)pyrene in rat and mouse liver Biochem Pharmacol., 1989; 38 (17): 2841–2847. doi: 10.1016/0006-2952(89)90439-5
18. Layeghkhavidaki H., Marie-Claire Lanhers M-C., Akbar S., Lynn G-P., Thierry O., Grova N., Appenzeller B., Jasniewski J., Feidt C., Corbier C., Yen F.T. Inhibitory action of benzo[α]pyrene on hepatic lipoprotein receptors in vitro and on liver lipid homeostasis in mice. PLoS One, 2014; 9 (7). e102991. doi: 10.1371/journal.pone.0102991
Review
For citations:
Polyakov L.M., Knyazev R.A., Trifonova N.V., Kotova M.V., Solovyova E.I., Ryabchenko A.V. Effect of blood plasma lipoproteins on hydroxylation of benzo[a]pyrene in liver microsomes of rats. Ateroscleroz. 2020;16(4):13-17. (In Russ.) https://doi.org/10.15372/ATER20200402
Views:
200
Email this article 