The cardiotonic properties of human apolipoprotein A-I
Abstract
The aim of this study was to determine the action of apoliporotein A-I (apoA-I) - the main protein of plasma high density lipoproteins on the work capacity of isolated rat heart and the influence on its effects of classic cardiotoinc drugs (adrenaline, digoxin) and nonselective β-blocker propranolol. Results. By the regime of the recirculation the physiological concentration of apoA-I (20 μg/ml) has stably elevated the intraventricular pressure but the coronary flow was changed insignificantly. Adrenaline has induced two-stage effect: initially it increased the frequency and the force of heart contractions but the induced left ventricular pressure decreased significantly. In the experiments with the simultaneous common perfusion of adrenaline and apoA-I the increased working capacity of isolated heart was recorded during the whole time of observation (30 min). ApoA-I has eliminated the cardiotonc activity of digoxin, and digoxin has removed cardiotonc effect of apoA-I too, and indicators of cardiac activity turned to control parameters but coronary flow was decreased. ApoA-I didn’t abolish the negative chronotropic effect of propranolol but substantially (more 2 fold) increased the left ventricular pressure.
About the Authors
A. R. Kolpakov
Institute of Biochemistry; Novosibirsk State Medical University
Russian Federation
Russian Federation
630117
Timakov str., 2
63091
Krasny av., 52
Novosibirsk
R. A. Knyazev
Institute of Biochemistry
Russian Federation
Russian Federation
630117
Timakov str., 2
Novosibirsk
N. V. Trifonova
Institute of Biochemistry
Russian Federation
Russian Federation
630117
Timakov str., 2
Novosibirsk
L. M. Polyakov
Institute of Biochemistry
Russian Federation
Russian Federation
630117
Timakov str., 2
Novosibirsk
References
1. Zhang Q., Meng Z. The inotropic effects of ammonia on isolated perfused rat hearts and the mechanisms involve // J. Exp. Biol. 2011. Vol. 214. P. 4048–4054.
2. Szokodi I., Kinnunen P., Tavi P. et al. Evidence for cAMP-independent mechanisms mediating the effects of adrenomedullin, a new inotropic peptide // Circulation. 1998. Vol. 97. P. 1062–1070.
3. Панин Л. Е. Влияние кортикостерона и липопротеинов плазмы на работоспособность и ультраструктуру изолированного сердца крыс / Л. Е. Панин, А. Р. Колпаков, В. Ф. Максимов // Кардиология. – 2007. – Т. 47, № 1. – С. 31–36.
4. Панин Л. Е. Кардиотонические свойства липопротеинов высокой плотности / Л. Е. Панин [и др.] // Атеросклероз. – 2013. – Т. 9, № 2. – С. 5–10.
5. Calabresi L., Rossoni G., Gomaraschi M. et al. High-density lipoproteins protect isolated rat hearts from ischemia-reperfusion injury by reducing cardiac tumor necrosis factor-alpha content and enhancing prostaglandin release // Circ. Res. 2003. Vol. 92 (3). P. 330–337.
6. Gomaraschi M., Calabresi L., Rossoni G. et al. Anti-inflammatory and cardioprotective activities of synthetic high-density lipoprotein containing apolipoprotein A-I mimetic peptides // J. Pharmacol. Exp. Ther. 2008. Vol. 324 (2). P. 776–783.
7. Алюхин Ю. С. Энергетика сердца и температурная адаптация организма / Ю. С. Алюхин // Физиол. журн. СССР. – 1975. – Т. 16, № 5. – С. 749–757.
8. Kang W., Weiss M. Digoxin uptake, receptor heterogeneity, and inotropic response in the isolated rat heart: a comprehensive kinetic model // J. Pharmacol. Exp. Ther. 2002. Vol. 302 (2). P. 577–582.
9. Iwai T., Tanonaka K., Kasahara S. et al. Protective effect of propranolol on mitochondrial function in the ischaemic heart // J. Pharmacol. 2002. Vol. 136 (3). P. 472–480.
10. Поляков Л. М. Определение апопротеина А-I методом иммуноферментного анализа / Л. М. Поляков, О. Н. Потеряева, Л. Е. Панин // Вопр. мед. химии. – 1991. – Т. 37, № 1. – С. 89–92.
11. Yates J. C., Beamish R. E., Dhalla N. S. Ventricular dysfunction and necrosis produced by adrenochrome metabolite of epinephrine: relation to pathogenesis of catecholamine cardiomyopathy // Am. Heart J. 1981. Vol. 102 (2). P. 210–221.
12. Поляков Л. М. Липопротеины высокой плотности и аполипопротеин А-I: регуляторная роль и новые терапевтические стратегии лечения атеросклероза / Л. М. Поляков, Л. Е. Панин // Атеросклероз. – 2013. – Т. 9, № 1. – С. 42–53.
Review
For citations:
Kolpakov A.R., Knyazev R.A., Trifonova N.V., Polyakov L.M. The cardiotonic properties of human apolipoprotein A-I. Ateroscleroz. 2015;11(4):20-24. (In Russ.)
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