aterosklerozАтеросклерозAteroscleroz2078-256X2949-3633НИИТПМ-филиал ИЦиГ СО РАН10.52727/2078-256X-2023-19-2-84-92ateroskleroz-902Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESАссоциации полиморфизмов некоторых генов с кардиометаболическими факторами риска у подростков НовосибирскаAssociations of polymorphisms of some genes with cardiometabolic risk factors in adolescents from Novosibirskhttps://orcid.org/0000-0002-2470-2133ДенисоваД. В.DenisovaD. V.

Диана Вахтанговна Денисова, д-р мед. наук, главный научный сотрудник лаборатории профилактическоймедицины

630089, г. Новосибирск, ул. Бориса Богаткова, 175/1

Diana V. Denisova, doctor of medical sciences, chief researcher of the laboratory of preventive medicine

175/1, Boris Bogatkov str., Novosibirsk, 630089

denisovadiana@gmail.comhttps://orcid.org/0000-0003-1547-624XГуражеваА. А.GurazhevaA. A.

Анна Александровна Гуражева, научный сотрудник лаборатории молекулярно-генетических исследований терапевтических заболеваний

630089, г. Новосибирск, ул. Бориса Богаткова, 175/1

Anna A. Gurazheva, researcher, laboratory of molecular genetic research of therapeutic diseases

175/1, Boris Bogatkov str., Novosibirsk, 630089

annapalna1@mail.ruhttps://orcid.org/0000-0002-7165-4496МаксимовВ. Н.MaximovV. N.

Владимир Николаевич Максимов, д-р мед. наук, проф., зав. лабораторией молекулярно-генетических исследований терапевтических заболеваний

630089, г. Новосибирск, ул. Бориса Богаткова, 175/1

Vladimir N. Maximov, doctor of medical sciences, professor, head. laboratory of molecular genetic research of therapeutic diseases

175/1, Boris Bogatkov str., Novosibirsk, 630089

medik11@mail.ruНаучно-исследовательский институт терапии и профилактической медицины – филиал Федерального государственного бюджетного научного учреждения «Федеральный исследовательский центр Институт цитологии и генетики Сибирского отделения Российской академии наук»Research Institutе of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences2023090720231928492Copyright © Денисова Д.В., Гуражева А.А., Максимов В.Н., 20232023Денисова Д.В., Гуражева А.А., Максимов В.Н.Denisova D.V., Gurazheva A.A., Maximov V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ateroskleroz.elpub.ru/jour/article/view/902

Цель исследования – изучить ассоциации полиморфизмов ряда генов с избыточным весом и некоторыми антропометрическими и биохимическими показателями в популяционной выборке подростков Новосибирска. Материал и методы. В 2019 г. в Новосибирске проведен популяционный скрининг репрезентативной выборки подростков (609 человек). Все дети и их родители подписали информированное согласие на обследование. Исследование одобрено локальным этическим комитетом НИИ терапии и профилактической медицины – филиала Института цитологии и генетики СО РАН. В ходе обследования заполнялся опросник, проводились антропометрические измерения, забор крови с последующим биохимическим исследованием. Для молекулярно-генетического анализа по таблицам случайных чисел отобрано 157 человек (75 мальчиков (47,8 %), 82 девочки (52,2 %)). Весовой статус подростков оценивался с помощью критериев IOTF (International Obesity Task Force). В последующем анализе сравнивались две группы: в группу 1 вошли лица с дефицитом веса и нормальной массой тела, в группу 2 – с избыточной массой тела, ожирением и экстремальным ожирением. Результаты. В общей группе различия у носителей разных генотипов rs9939609 гена FTO найдены по уровню холестерина липопротеинов низкой плотности (ХС ЛПНП) (р = 0,024). У девочек с генотипом АА rs9939609 обнаружена наибольшая толщина кожной складки в средней трети правого плеча. Выявлены различия у носителей разных генотипов rs7903146 гена TCF7L2 по уровню глюкозы в плазме крови натощак (ГПН) (р = 0,021). Толщина кожной складки в средней трети правого плеча оказалась больше у девочек с генотипом СС по сравнению с носителями генотипов СТ и ТТ (р = 0,041). В отдельных подгруппах у носителей разных генотипов rs10811661 гена CDKN2AB найдены различия по содержанию ХС липопротеинов высокой плотности, ГПН и окружности талии, у носителей вариантов нуклеотидной последовательности (ВНП) rs2237892 гена KCNQ1 – по концентрации триглицеридов, ГПН, ХС ЛПНП и общего ХС, у носителей ВНП rs1111875 гена HHEX – по уровню общего ХС, ХС ЛПНП, окружности талии и бедер, толщине кожной складки под лопаткой, диастолическому артериальному давлению (ДАД). Заключение. Обнаружены ассоциации изучаемых ВНП (rs9939609, rs7903146, rs10811661, rs2237892, rs1111875) в группе в целом и в отдельных под- группах (с разделением по индексу массы тела (ИМТ), полу), с антропометрическими и биохимическими показателями, такими как содержание общего ХС, триглицеридов, ХС ЛПНП, ГПН, ДАД, окружность талии и бедер, толщина кожной складки под лопаткой и в средней трети правого плеча. Статистически значимых различий по частотам генотипов и аллелей изучаемых ВНП между 1-й и 2-й группами не получено.

Aim of the study was to investigate the association of polymorphisms of some genes with overweight and certain anthropometric and biochemical parameters in a population sample of adolescents in Novosibirsk. Material and methods. In 2019, a population-based screening of a representative sample of adolescents (609 people) was carried out in Novosibirsk. All children and their parents signed an informed consent for the examination. The study was approved by the local Ethics Committee of the Research Institute of Internal and Preventive Medicine – Branch of the Institute of Cytology and Genetics of SB RAS. During the examination, a questionnaire was filled out, anthropometric measurements were carried out, blood sampling was carried out, followed by a biochemical study. 157 people (75 boys (47.8 %), 82 girls (52.2 %)) were selected for molecular genetic analysis using tables of random numbers. The weight status of adolescents was assessed using the IOTF (International Obesity Task Force) criteria. The subsequent analysis compared 2 groups: 1st group included teenagers with weight deficiency and normal body weight), the 2nd – with overweight, obesity and extreme obesity). Results. In the general group, differences in carriers of different genotypes of the rs9939609 FTO gene were found in the level of low-density lipoprotein cholesterol (LDL-C) (p = 0,024). The girls with the AA rs9939609 genotype had the greatest average thickness of the skin fold in the middle third of the right shoulder. Differences were found in carriers of different genotypes rs7903146 of TCF7L2 gene in the average fasting plasma glucose (FPG) level (p = 0,021). The average thickness of the skin fold in the middle third of the right shoulder was greater in girls carrying the CC genotype compared with carriers of the CT and TT genotypes (p = 0,041). In separate subgroups, in carriers of different genotypes of rs10811661 CDKN2AB gene were found differences in high-density lipoprotein cholesterol, FPN, and waist circumference, in nucleotide sequence variant (NSV) rs2237892 KCNQ1 gene carriers – in triglyceride, FPG, LDL-C and total cholesterol concentration, in rs1111875 HHEX gene carriers – in total cholesterol, LDL-C level, waist and hip circumference, thickness of the skin fold under the scapula, diastolic blood pressure. Conclusions. Associations of the studied NSV (rs9939609, rs7903146, rs10811661, rs2237892, rs1111875) were found in the group as a whole and in separate subgroups (with division by body mass index, gender), with anthropometric and biochemical parameters, such as total cholesterol, triglyceride, LDL-C, FPN content, diastolic blood pressure, waist and hip circumferences, thickness of the skin fold under the scapula and in the middle third of the right shoulder. There were no statistically significant differences in the frequencies of studied NVS genotypes and alleles between the 1st and 2nd groups.

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The authors declare that there are no conflicts of interest present.