aterosklerozАтеросклерозAteroscleroz2078-256X2949-3633НИИТПМ-филиал ИЦиГ СО РАНateroskleroz-724Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESГенетика долголетия: длина теломер лейкоцитов как маркер старения и фактор риска развития возраст-зависимых заболеваний у человекаGenetics of longevity: telomere length of leukocytes as a marker of aging and a risk factor for age-related diseases in humansМаксимовВ. Н.MaksimovV. N.

Владимир Николаевич Максимов, д-р мед. наук, зав. лабораторией

СО РАМН

ФГБУ «НИИ терапии и профилактической медицины»

лаборатория молекулярно-генетических исследований терапевтических заболеваний

ул. Бориса Богаткова, 175/1

Новосибирск

SB RAMS

Research Institute of Internal and Preventive Medicine

630089

Boris Bogatkov str., 175/1

Novosibirsk

medik11@mail.ruВоропаеваЕ. Н.VoropaevaE. N.

Елена Николаевна Воропаева, научный сотрудник

СО РАМН

ФГБУ «НИИ терапии и профилактической медицины»

лаборатория молекулярно-генетических исследований терапевтических заболеваний

630089

ул. Бориса Богаткова, 175/1

Новосибирск

SB RAMS

Research Institute of Internal and Preventive Medicine

630089

Boris Bogatkov str., 175/1

Novosibirsk

vena.81@mail.ruБобакМ.BobakM.

Мартин Бобак, д-р философии, проф. эпидемиологии, зам. руководителя отдела

отдел эпидемиологии и здоровья

1–19 Торрингтон Плейс WC1EGBT

Лондон

1–19 Torrington Place

London

m.bobak@ucl.ac.ukМалютинаС. К.MalyitinaS. K.

Софья Константиновна Малютина, д-р мед. наук, проф., зав. лабораторией

СО РАМН

ФГБУ «НИИ терапии и профилактической медицины»

лаборатория этиопатогенеза и клиники внутренних заболеваний

630089

ул. Бориса Богаткова, 175/1

Новосибирск

SB RAMS

Research Institute of Internal and Preventive Medicine

630089

Boris Bogatkov str., 175/1

Novosibirsk

smalyutina@hotmail.comВоеводаМ. И.VoevodaM. I.

Михаил Иванович Воевода, д-р мед. наук, проф., член-корр., директор

СО РАМН

ФГБУ «НИИ терапии и профилактической медицины»

630089

ул. Бориса Богаткова, 175/1

Новосибирск

SB RAMS

Research Institute of Internal and Preventive Medicine

630089

Boris Bogatkov str., 175/1

Novosibirsk

mvoevoda@ya.ruРАМНRAMSУниверситетский колледж ЛондонаUniversity College London20141404202210459Copyright © Максимов В.Н., Воропаева Е.Н., Бобак М., Малютина С.К., Воевода М.И., 20222022Максимов В.Н., Воропаева Е.Н., Бобак М., Малютина С.К., Воевода М.И.Maksimov V.N., Voropaeva E.N., Bobak M., Malyitina S.K., Voevoda M.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://ateroskleroz.elpub.ru/jour/article/view/724

   Статья посвящена обзору исследований в области генетики долголетия (современное состояние), в частности – использованию измерения длины теломер лейкоцитов в качестве маркера старения и индикатора риска развития возраст-зависимых заболеваний у человека. Представлен обзор современных лабораторных методов оценки длины теломер. В рамках пилотного этапа проекта по изучению биомаркеров «биологического» возраста в российской популяции (грант РНФ) выполнено тестирование  метода с помощью количественной полимеразной цепной реакции в реальном времени на основе методики Cawthon (2002 г.). Результаты тестирования методики удовлетворительные, воспроизводимость методики составила 95,5 %, отработанный подход будет использован для анализа в настоящем исследовании.

   The manuscript is devoted to review in the area of genetics of longevity (current state of knowledge), in particular, the use of leukocyte telomere length measurement as a marker of aging and as an indicator of the risk for age-related diseases in humans. The paper also gives an overview of laboratory methods to assess telomere length. In the frame of pilot study of the project on biomarkers of «biological age» in Russian population (grant RCSF) the method for analysis of telomeres length by real-time PCR on the base of technique by Cawthon (2002) was tested. The results of testing provided required accuracy, reproducibility of method’s was of 95,5 %, the tried and tested technique will be used for analysis in present project.

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The authors declare that there are no conflicts of interest present.