aterosklerozАтеросклерозAteroscleroz2078-256X2949-3633НИИТПМ-филиал ИЦиГ СО РАНateroskleroz-596Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПолиморфизм генов белков – регуляторов воспаления при атеросклерозе, осложненном развитием острого инфаркта миокардаGenes polymorphism of proteins – regulators – of the inflammation at atherosclerosis complicated with development of myocardium infarctКоненковВ. И.KonenkovV. I.

Владимир Иосифович Коненков, д-р мед. наук, проф., академик, директор

СО РАМН

Учреждение Российской академии медицинских наук Научно-исследовательский институт клинической и экспериментальной лимфологии

лаборатория клинической иммуногенетики

630117

ул. Арбузова, 6

Новосибирск

SB RAMS

Institution of the Russian Academy of Medical Sciences Research Institute of Clinical and Experimental Lymphology

laboratory of clinical immunogenetics

630117

Arbuzova str., 6

Novosibirsk

konenkov@soramn.ruШевченкоА. В.ShevchenkoA. V.

Алла Владимировна Шевченко, канд. биол. наук, с. н. с. 

СО РАМН

Учреждение Российской академии медицинских наук Научно-исследовательский институт клинической и экспериментальной лимфологии

лаборатория клинической иммуногенетики

630117

ул. Арбузова, 6

Новосибирск

SB RAMS

Institution of the Russian Academy of Medical Sciences Research Institute of Clinical and Experimental Lymphology

laboratory of clinical immunogenetics

630117

Arbuzova str., 6

Novosibirsk

shalla64@mail.ruПрокофьевВ. Ф.ProkofievV. F.

Виктор Федорович Прокофьев, канд. мед. наук, в. н. с.

СО РАМН

Учреждение Российской академии медицинских наук Научно-исследовательский институт клинической и экспериментальной лимфологии

лаборатория клинической иммуногенетики

630117

ул. Арбузова, 6

Новосибирск

SB RAMS

Institution of the Russian Academy of Medical Sciences Research Institute of Clinical and Experimental Lymphology

laboratory of clinical immunogenetics

630117

Arbuzova str., 6

Novosibirsk

vprok@ngs.ruВоеводаМ. И.VoevodaM. I.

Михаил Иванович Воевода, д-р мед. наук, проф., член-корр., директор

СО РАМН

Учреждение Российской академии медицинских наук Научно-исследовательский институт терапии

630089

ул. Б. Богаткова, 175/1

Новосибирск

SB RAMS

Establishment of the Russian Academy of Medical Sciences Research Institute of Therapy

630089

B. Bogatkova str., 175/1

Novosibirsk

voevoda@iimed.ruРАМНРоссияRAMSRussian Federation20112803202271518Copyright © Коненков В.И., Шевченко А.В., Прокофьев В.Ф., Воевода М.И., 20222022Коненков В.И., Шевченко А.В., Прокофьев В.Ф., Воевода М.И.Konenkov V.I., Shevchenko A.V., Prokofiev V.F., Voevoda M.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://ateroskleroz.elpub.ru/jour/article/view/596

   С целью исследования характера ассоциированности генотипов цитокинов и ряда других регуляторных белков с развитием острого инфаркта миокарда (ОИМ) у мужчин разного возраста исследован полиморфизм промоторных участков генов: TNFC-863A; TNFG-308A; TNFG-238A; IL-1 β C-511T; IL-1 β T-31C; IL-4C-590T; IL-6G-174C; IL-10А-1082G; IL-10C-590A; MMP-2C-1306T; MMP-9C-1562T; HSP-70-2 A-1267G и HSP-70-HOM C- 2437T. Генотипирование аллельных вариантов генов осуществляли методом рестриктного анализа продуктов амплификации. Полиморфные участки амплифицировали с использованием пары специфичных праймеров, продукты амплификации подвергались гидролизу соответствующими эндонуклеазами рестрикции, гель – электрофорезу с последующим документированием и анализом. Обследовано 223 мужчины, перенесшие ранее ОИМ, и 95 здоровых мужчин европеоидного происхождения, соответствующих возрасту и социально бытовым условиям жизни. Показано, что практически в каждом полиморфном участке исследованных генов выявляются аллели и генотипы, в различной степени ассоциированные с развитием ОИМ. Часто эти генетические факторы риска синергически взаимодействуют с такими классическими факторами риска развития атеросклероза, как курение, возраст, избыточный индекс массы тела, повышенное артериальное давление. Установлено, что комплексный анализ выявления в геноме пациента целого ряда генотипов полиморфных участках генов цитокинов IL-1β – IL-6 – IL-4 – IL-10 – TNFA позволяет получить значительно более высокие показатели величины отношения шансов. Исследование полиморфизма генов белков, принимающих участие в регуляции активности воспаления, имеет самостоятельное значение в разработке комплексных генетических факторов риска развития атеросклероза и его сосудистых осложнений.

   Promoter polymorphism TNFC-863A; TNFG-308A; TNFG-238A; IL-1 β C-511T; IL-1 β T-31C; IL4C-590T; IL-6G-174C; IL10А-1082G; IL10C-590A; MMP-2C-1306T; MMP-9C-1562 T; HSP-70-2 A-1267G and HSP-70-HOM C-2437T of genes was investigated with the purpose of research association their genotypes with development of myocardium infarct (MI) at different age of means. For genotyping we used a restriction fragment length polymorphism (RFLP) method of PCR pro ducts (RFLP-analysis) by specific primers. The amplification products were hydrolysis and restriction fragments were analysis. We are investigation 223 patients and 95 healthy men. It Is shown, that practically in each polymorphic site of the investigated genes come to light genotypes, in a various degree associated with development MI. Frequently these genetic risk factors was cooperate with classical risk factors of atherosclerosis development: smoking, age, weight index of the body, arterial pres sure. Complex analysis a lot of genotypes polymorphic sites of genes IL-1β – IL-4- IL-6-IL-10 – TNF-A are show higher parameters of relation risk (OR). Polymorphism genes research of proteins which participating in regulation of inflammation activity is independent value in creation of complex genetic risk factors of development of an atherosclerosis and its vascular complications.

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The authors declare that there are no conflicts of interest present.