aterosklerozАтеросклерозAteroscleroz2078-256X2949-3633НИИТПМ-филиал ИЦиГ СО РАН10.15372/ATER20190304ateroskleroz-241Research ArticleОРИГИНАЛЬНЫЕ СТАТЬИORIGINAL ARTICLESПИЛОТНОЕ ИССЛЕДОВАНИЕ АССОЦИАЦИИ ПОЛИМОРФИЗМОВ ГЕНОВ TRPA1 И TRPV1 С ИНФАРКТОМ МИОКАРДАPILOT STUDY OF THE ASSOCIATION OF TRPA1 AND TRPV1 GENE POLYMORPHISMS WITH MYOCARDIAL INFARCTIONОрловП. С.OrlovP. S.МаксимовВ. Н.MaksimovV. N.МихайловаС. В.MikhaylovaS. V.ИванощукД. Е.IvanoshchukD. E.МалютинаС. К.MalyutinaS. K.ВоеводаМ. И.VoevodaM. I.НИИ терапии и профилактической медицины – филиал ФГБНУ ФИЦ Институт цитологии и генетики СО РАН; ФГБНУ ФИЦ Институт цитологии и генетики СО РАН; ФГАОУ ВО Новосибирский национальный исследовательский государственный университетРоссияResearch Institute of Therapy and Preventive Medicine – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS; Federal Research Center Institute of Cytology and Genetics of SB RAS; Novosibirsk State UniversityRussian FederationФГБНУ ФИЦ Институт цитологии и генетики СО РАНРоссияFederal Research Center Institute of Cytology and Genetics of SB RASRussian FederationНИИ терапии и профилактической медицины – филиал ФГБНУ ФИЦ Институт цитологии и генетики СО РАНРоссияResearch Institute of Therapy and Preventive Medicine – Branch of Federal Research Center Institute of Cytology and Genetics of SB RASRussian FederationНИИ терапии и профилактической медицины – филиал ФГБНУ ФИЦ Институт цитологии и генетики СО РАН; ФГБНУ ФИЦ Институт цитологии и генетики СО РАН; ФГАОУ ВО Новосибирский национальный исследовательский государственный университет; ФГБНУ ФИЦ фундаментальной и трансляционной медициныРоссияResearch Institute of Therapy and Preventive Medicine – Branch of Federal Research Center Institute of Cytology and Genetics of SB RAS; Federal Research Center Institute of Cytology and Genetics of SB RAS; Novosibirsk State University; Federal Research Center for Fundamental and Translational MedicineRussian Federation2019221220191535055Copyright © Орлов П.С., Максимов В.Н., Михайлова С.В., Иванощук Д.Е., Малютина С.К., Воевода М.И., 20192019Орлов П.С., Максимов В.Н., Михайлова С.В., Иванощук Д.Е., Малютина С.К., Воевода М.И.Orlov P.S., Maksimov V.N., Mikhaylova S.V., Ivanoshchuk D.E., Malyutina S.K., Voevoda M.I.This work is licensed under a Creative Commons Attribution 4.0 License.https://ateroskleroz.elpub.ru/jour/article/view/241

Цель работы – исследовать ассоциацию однонуклеотидных полиморфизмов (ОНП) генов TRPA1 (rs13268757) и TRPV1 (rs222747) с инфарктом миокарда.

Материал и методы

Материал и методы. Группы инфаркта миокарда (200 человек) и контроля (420 человек) сформированы в рамках работы по международному проекту HAPIEЕ. Генотипирование групп по исследуемым полиморфизмам rs13268757 и rs222747 выполнено методом полимеразной цепной реакции в реальном времени на приборе «StepOnePlus» («Applied Biosystems», США) с использованием TaqMan зондов («Applied Biosystems») согласно стандартному протоколу. Межгрупповое сравнение частот аллелей/генотипов каждого из изученных полиморфизмов проводили с использованием точного критерия Фишера. Соответствие частот генотипов равновесию Харди – Вайнберга проверяли с использованием метода χ2. Относительный риск инфаркта миокарда (ИМ) по конкретному аллелю или генотипу вычисляли как отношение шансов (OR, odds ratio) с использованием точного двух- стороннего критерия Фишера и критерия χ2 Пирсона. Различия считали статистически достоверными при уровне значимости p < 0,05.

Результаты

Результаты. Частота гомозиготного генотипа АА по- лиморфизма rs13268757 гена TRPA1 у пациентов с ИМ была достоверно выше, чем у лиц группы контроля (GG + AG), OR = 2,621 (95%-й ДИ 1,112–6,175; p = 0,034). Для rs222747 TRPV1 статистически значимых различий не получено.

Заключение

Заключение. Впервые в России проведена проверка полиморфизма rs222747 гена TRPV1 и полиморфизма rs13268757 гена TRPA1 на ассоциацию с ИМ. Для полиморфизма rs13268757 гена TRPA1 установлена ассоциация с ИМ.

The aim of the study was to investigate the association of SNPs in the TRPA1 (rs13268757) and TRPV1 (rs222747) genes with myocardial infarction.

Material and methods

Material and methods. Myocardial infarction (MI) (200 individuals) and control (420 individuals) groups were formed as a part of international HAPIEE project. Genotyping the groups for the studied rs13268757 and rs222747 polymorphisms was performed by a real-time PCR on a StepOnePlus device (Applied Biosystems, USA) using TaqMan probes (Applied Biosystems, USA) according to a standard protocol. Intergroup comparison of the allele/genotype frequencies for each of the studied polymorphisms was calculated using Fisher's exact test and the SPSS 11.0 program. The correspondence of genotype frequencies to Hardy-Weinberg equilibrium was tested using the χ2 method. The relative risk of MI for a particular allele or genotype was calculated as an odds ratio (OR) using Fisher’s exact two-sided test and Pearson chi-square test. Differences were considered statistically significant with a significance level p <0.05.

Results

Results. The frequency of the homozygous AA genotype for the TRPA1 gene rs13268757 polymorphism differed significantly in patients with MI as compared with the control, AA vs GG + AG OR = 2.621 (95 % CI 1.112–6.175; p = 0.034). No statistically significant results were shown for the TRPV1 rs222747.

Conclusion

Conclusion. The association of the TRPV1 gene rs222747 and TRPA1 gene rs13268757 polymorphisms with MI was for the first time checked in Russia. The association with MI was shown for the TRPA1 gene rs13268757 polymorphism.

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The authors declare that there are no conflicts of interest present.